A recent approach of generating 3D brain tissues, namely “cerebral organoids”, closely reproduces the endogenous developmental program. This approach can give rise to retinal identities, ventral telencephalon, developing cerebral cortex, and choroid plexus, within 1–2 months (Lancaster and Knoblich, 2014). Recent development of these 3D brain organoids derived from human iPSCs is a promising technology for understanding the development of in vitro disease models and investigating in particular the human polygenic disorders where animal models are not sufficient (Lindborg et al., 2016; Quadrato et al., 2016).
