Mice were allowed to acclimate to the test room for at least 1 h before experimental manipulation. Prior to testing, each mouse was weighed and injected intraperitoneally (i.p.) with drug or vehicle. For the diazepam and THC experiments, pretreatment time was 1 h. For the JZL184 and PF-3845 experiments, pretreatment time was 2 h, based on previous reports that endocannabinoid levels peak 2 h after treatment with either compound (Ahn et al., 2009; Long et al., 2009). The selective CB1 receptor antagonist rimonabant (0.3 mg/kg, i.p.) was administered 10 min prior to JZL184 or PF-3845 treatment. This dose of rimonabant was chosen based on pilot data, which indicated deficits in locomotor activity at higher doses.
