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Chunk #6 — 2. Materials and Methods — 2.2 Drugs

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Inhibition of endocannabinoid catabolic enzymes elicits anxiolytic-like effects in the marble burying assay.
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Diazepam (DZ) was purchased from Sigma-Aldrich (St. Louis, MO). Rimonabant (Rim) and Δ9-tetrahydrocannabinol (THC) were obtained from the National Institute on Drug Abuse (Bethesda, MD). The MAGL inhibitor JZL184 (Long et al., 2009) and the FAAH inhibitor PF-3845 (Ahn et al., 2009) were synthesized as described previously. All drugs were dissolved in a vehicle consisting of equal parts ethanol and Alkamuls-620 (Rhone-Poulenc, Princeton, NJ), then diluted with normal saline to a final ratio of 1:1:18. All compounds were administered intraperitoneally (i.p.), at a volume of 10 μl/g body mass. All solutions were warmed to room temperature prior to injection.