Subjects consisted of male C57BL/6J mice (Jackson Laboratory, Bar Harbor, ME) that were approximately 10 weeks of age and weighed approximately 25 g at the beginning of the study. Mice were group housed 4-6 per cage in a temperature (20-22° C) and humidity controlled, AAALAC-approved facility maintained on a 12:12 light:dark cycle and were provided with ad libitum access to food and water. Mice were randomly assigned to treatment groups. All experiments were approved by the Institutional Animal Care and Use Committee at Virginia Commonwealth University.
