Chunk #65 — II. Samples for genome studies of human addiction vulnerabilities and related phenotypes (Figure 2)

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Molecular genetics of addiction and related heritable phenotypes: genome-wide association approaches identify "connectivity constellation" and drug target genes with pleiotropic effects.

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For genotyping, DNAs were carefully quantitated and combined into 12 pools that each represented about 20 subjects, based on estimates for frontal brain corrected for total cranial volume. We thus constructed 4 DNA pools from individuals with the lowest, 4 pools from individuals with intermediate, and 4 pools from individuals with the highest estimates of total frontal brain volumes and subjected these DNA pools to Affymetrix 500k genotyping as noted above. t tests compared differences between highest and lowest brain volume groups. SNPs that displayed nominally-significant t values and that also displayed rank order of allelic frequencies with either highest tercile > intermediate tercile > lowest tercile or the converse are included in these analyses.