Because alterations in glucocorticoid receptor 1F activity could be derived from nucleotide sequence variation and/or epigenetic modifications, we sequenced the NR3C1 promoter region from each subject. No sequence variation was seen among subjects and all of the sequences were identical to those published previously19. Moreover, for each subject, the genomic sequences targeted for binding by the primers used for bisulfite mapping were identical to the published sequence19, thus eliminating potential primer bias between subjects in sodium bisulfite mapping.
