Chunk #23 — RESULTS — DG-like hippocampal neurons derived from BD patients are hyperexcitable, but the intrinsic properties of LR and NR neurons are profoundly different
We patch clamped Prox1∷EGFP-expressing neurons at 3.5 weeks postdifferentiation from four control cell lines (95 cells), three LR patient cell lines (84 cells) and three NR patient cell lines (63 cells). Representative traces of evoked action potentials of control, LR and NR neurons are shown in Figure 2a–c. The neurons derived from both LR and NR patient cells were hyperexcitable, with an average of 21.2 ± 2.4 (control), 48 ± 5.5 (LR) and 48.7 ± 8.3 (NR) evoked action potentials (P<0.0001 for LR neurons vs control and P = 0.0002 for NR neurons vs control), producing more action potentials with injection of current (Figure 2d with a patient-by-patient plot in Figure 2e). The spontaneous firing rate was the highest in the LR group and lowest in the control group, with averages of 0.71 ± 0.14 Hz (LR), 0.35 ± 0.08 Hz (NR) and 0.24 ± 0.06 Hz (control) firing rates (Figure 2f, P = 0.0006 LR vs control, P = 0.28 NR vs control and P = 0.03 LR vs NR). Averaging all BD lines together and comparing them with
