Chunk #24 — RESULTS — DG-like hippocampal neurons derived from BD patients are hyperexcitable, but the intrinsic properties of LR and NR neurons are profoundly different
Hz (NR) and 0.24 ± 0.06 Hz (control) firing rates (Figure 2f, P = 0.0006 LR vs control, P = 0.28 NR vs control and P = 0.03 LR vs NR). Averaging all BD lines together and comparing them with the control lines revealed a significance of P = 0.0055 BD vs control). Using voltage clamp mode, we recorded sodium and potassium currents. Representative traces are shown in Supplementary Figures 1c–e. The averages of the sodium currents are shown in Figure 2g; the fast and slow potassium currents are shown in Supplementary Figures 1a and b. We found that LR and control neurons exhibited similar sodium currents, but NR sodium currents were 45% lower (Figure 2g). There was no significant change in the potassium currents (Supplementary Figures 1a and b). The ratio between the sodium and potassium currents (see Materials and Methods) was 25% higher in LR (not significant) compared with control neurons and 46% lower (P = 0.0006) in NR neurons compared with control neurons (Figure 2h).
