Chunk #25 — RESULTS — DG-like hippocampal neurons derived from BD patients are hyperexcitable, but the intrinsic properties of LR and NR neurons are profoundly different
Spike shape is an important determinant of neuronal excitability. We therefore analyzed the spike shape for the three groups. Figure 2j–l displays representative traces of an action potential of LR, NR and control DG-like neurons recorded in current clamp mode. Figure 2i gives a schematic of the parameters analyzed for the spike shape. LR neuron spikes had increased amplitude of 20% (P = 0.0006) compared with control neurons, whereas NR neuron spikes had 15% lower amplitude than control neurons (P = 0.01) (P<0.0001 between LR and NR) (Supplementary Figure 1f). It should be noted that the spike height is correlated with the size of sodium currents, but this does not explain all the differences between subjects. Other factors that have an impact on the spike height are the sodium channels kinetics, the potential in which they open and the potential in which the potassium channels open. In addition, there are more factors, for example, the sodium currents are increased (at −40 mV, there is ~ 80%, P = 0.15 more sodium currents in the LR neurons than the controls), additionally
