H1-derived iN cells were dissociated using Enzyme-Free Cell Dissociation Buffer (Gibco) 7 days after infection (i.e., on day 6) without co-culture of astrocytes, and 105 cells were unilaterally injected under hypothermia-induced anesthesia into the striatum of postnatal day 2 NOD-SCID; IL2Rγ knock-out mice. Mice were processed for immunocytochemistry or slice electrophysiology six weeks after transplantation.
