Chunk #41 — EXPERIMENTAL PROCEDURES — Channelrhodopsin experiments

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Rapid single-step induction of functional neurons from human pluripotent stem cells.

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H1 cells were co-infected with viruses expressing Ngn2 and ChiEF2-tdTomato on day 1, and mouse cortical neurons were added for co-culture on day 3. iN cells were analyzed at day 21 as described in detail in the Supplemental Methods.