Chunk #40 — EXPERIMENTAL PROCEDURES — RNAi experiments

Source

Rapid single-step induction of functional neurons from human pluripotent stem cells.

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Ca2+-imaging experiments were performed with iN cells that were infected with a lentivirus expressing GCaMP6M on day 3 after induction, co-cultured with mouse cortical neurons at day 6 after induction, and analyzed at day 21. See Supplemental Materials for details.