Western blot and RNA FISH assays demonstrated that YY2 was upregulated in both glioma tissues and cells (Figures 4A and 4B; Figure S2D). Moreover, high expression of YY2 was positively associated with poor prognosis in patients with glioma (Figure S1D). Glioma cells with YY2 overexpression also showed a significantly increased proliferation rate compared with that of the cells transfected with the NC vector (Figure 4C). Moreover, overexpression of YY2 significantly inhibited the apoptosis of glioma cells, whereas knockdown of YY2 promoted apoptosis (Figure 4D). Similarly, overexpression of YY2 significantly increased the numbers of migrating and invading glioma cells, which were reduced with YY2 knockdown (Figure 4E).
