In the prefrontal cortex, handling increased 2-AG (Handling main effect: F1,26 = 22.51, P = 0.000; η2 = 0.445; Fig. 6b), but not anandamide (P > 0.05; Fig. 6c) levels relative to non-handling. In the piriform cortex, handling decreased anandamide (Handling main effect: F1,28 = 4.83, P = 0.036; η2 = 0.129; Fig. 6f), but not 2-AG (P > 0.05; Fig. 6e) levels relative to non-handling. In the nucleus accumbens, handling increased anandamide (Handling main effect: F1,28 = 5.03, P = 0.033; η2 = 0.150; Fig. 6i) levels, without altering levels of 2-AG (P = 0.109; Fig. 6h), relative to non-handling. In the hippocampus, endocannabinoid levels were not affected by handling (P > 0.05; Fig. 6j–l).
