How to culture, record and stimulate neuronal networks on micro-electrode arrays (MEAs).
- Authors
- Hales, Chadwick M; Rolston, John D; Potter, Steve M
- Year
- 2010
- Journal
- Journal of visualized experiments : JoVE
- PMID
- 20517199
- DOI
- 10.3791/2056
- PMCID
- PMC3152853
For the last century, many neuroscientists around the world have dedicated their lives to understanding how neuronal networks work and why they stop working in various diseases. Studies have included neuropathological observation, fluorescent microscopy with genetic labeling, and intracellular recording in both dissociated neurons and slice preparations. This protocol discusses another technology, which involves growing dissociated neuronal cultures on micro-electrode arrays (also called multi-electrode arrays, MEAs). There are multiple advantages to using this system over other technologies. Dissociated neuronal cultures on MEAs provide a simplified model in which network activity can be manipulated with electrical stimulation sequences through the array's multiple electrodes. Because the network is small, the impact of stimulation is limited to observable areas, which is not the case in intact preparations. The cells grow in a monolayer making changes in morphology easy to monitor with various imaging techniques. Finally, cultures on MEAs can survive for over a year in vitro which removes any clear time limitations inherent with other culturing techniques. Our lab and others around the globe are utilizing this technology to ask important questions about neuronal networks. The purpose of this protocol is to provide the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.
No entities extracted from this document yet.
No uploaded files.
In this knowledge base
| Title | Year | PMID |
|---|---|---|
| Genetics of Alcohol Use Disorder: A Role for Induced Pluripotent Stem Cells? | 2018 | 29897633 |
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Does neural computation feel like something? | Gidon A et al. | — | 2025 | → |
| Self-Organizing Neural Networks in Organoids Reveal Principles of Forebrain Circuit Assembly | Hernandez S et al. | — | 2025 | — |
| A comprehensive review of electrophysiological techniques in amyotrophic lateral sclerosis research. | Ren K et al. | — | 2024 | → |
| Decomposing past and future: Integrated information decomposition based on shared probability mass exclusions. | Varley TF | — | 2023 | → |
| Functional imaging of conduction dynamics in cortical and spinal axons. | Radivojevic M et al. | — | 2023 | → |
| Modular architecture facilitates noise-driven control of synchrony in neuronal networks. | Yamamoto H et al. | — | 2023 | → |
| SARS-CoV-2 spike protein reduces burst activities in neurons measured by micro-electrode arrays. | Salvador M et al. | — | 2023 | → |
| Focused ultrasound neuromodulation on a multiwell MEA. | Saccher M et al. | — | 2022 | → |
| Neurodevelopmental copy-number variants: A roadmap to improving outcomes by uniting patient advocates, researchers, and clinicians for collective impact. | Commission on Novel Technologies for Neurodevelopmental Copy Number Variants | — | 2022 | → |
| Self-organization of in vitro neuronal assemblies drives to complex network topology. | Antonello PC et al. | — | 2022 | → |
| 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics. | Shin H et al. | — | 2021 | → |
| The Type 2 Diabetes Factor Methylglyoxal Mediates Axon Initial Segment Shortening and Alters Neuronal Function at the Cellular and Network Levels. | Griggs RB et al. | — | 2021 | → |
| Defining parameters of specificity for bioluminescent optogenetic activation of neurons using in vitro multi electrode arrays (MEA). | Prakash M et al. | — | 2020 | → |
| Deletion of LRP1 From Astrocytes Modifies Neuronal Network Activity in an <i>in vitro</i> Model of the Tripartite Synapse. | Romeo R et al. | — | 2020 | → |
| Dextran-coated iron oxide nanoparticle-induced nanotoxicity in neuron cultures. | Badman RP et al. | — | 2020 | → |
| Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan alters the ratio of excitatory and inhibitory synapses. | Gottschling C et al. | — | 2019 | → |
| L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks. | Plumbly W et al. | — | 2019 | → |
| Novel roles of ER stress in repressing neural activity and seizures through Mdm2- and p53-dependent protein translation. | Liu DC et al. | — | 2019 | → |
| Advances in Nano Neuroscience: From Nanomaterials to Nanotools. | Pampaloni NP et al. | — | 2018 | → |
| Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells. | Shall G et al. | — | 2018 | → |
| Genetics of Alcohol Use Disorder: A Role for Induced Pluripotent Stem Cells? | Prytkova I et al. | — | 2018 | → |
| MicroRNA profiling during directed differentiation of cortical interneurons from human-induced pluripotent stem cells. | Tu J et al. | — | 2018 | → |
| Neurotropism and behavioral changes associated with Zika infection in the vector Aedes aegypti. | Gaburro J et al. | — | 2018 | → |
| Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells. | Cvetkovic C et al. | — | 2018 | → |
| Transparent poly(3,4-ethylenedioxythiophene)-based microelectrodes for extracellular recording. | Flachs D et al. | — | 2018 | → |
| Zika virus-induced hyper excitation precedes death of mouse primary neuron. | Gaburro J et al. | — | 2018 | → |
| Measures of spike train synchrony for data with multiple time scales. | Satuvuori E et al. | — | 2017 | → |
| Robot-Embodied Neuronal Networks as an Interactive Model of Learning. | Shultz AM et al. | — | 2017 | → |
| Understanding spatial and temporal patterning of astrocyte calcium transients via interactions between network transport and extracellular diffusion. | Shtrahman E et al. | — | 2017 | → |
| Combined Optogenetic and Chemogenetic Control of Neurons. | Berglund K et al. | — | 2016 | → |
| Criticality Maximizes Complexity in Neural Tissue. | Timme NM et al. | — | 2016 | → |
| Electrical Identification and Selective Microstimulation of Neuronal Compartments Based on Features of Extracellular Action Potentials. | Radivojevic M et al. | — | 2016 | → |
| How Microelectrode Array-Based Chick Forebrain Neuron Biosensors Respond to Glutamate NMDA Receptor Antagonist AP5 and GABA<sub>A</sub> Receptor Antagonist Musimol. | Kuang SY et al. | — | 2016 | → |
| Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial. | Huang T et al. | — | 2016 | → |
| Neural Substrate Expansion for the Restoration of Brain Function. | Chen HI et al. | — | 2016 | → |
| A low-noise, modular, and versatile analog front-end intended for processing in vitro neuronal signals detected by microelectrode arrays. | Regalia G et al. | — | 2015 | → |
| Closed-loop neuro-robotic experiments to test computational properties of neuronal networks. | Tessadori J et al. | — | 2015 | → |
| Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent optogenetic inhibition. | Tung JK et al. | — | 2015 | → |
| Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model. | Tedesco M et al. | — | 2015 | → |
| Optogenetic feedback control of neural activity. | Newman JP et al. | — | 2015 | → |
| Upward synaptic scaling is dependent on neurotransmission rather than spiking. | Fong MF et al. | — | 2015 | → |
| Micro and Nano-Scale Technologies for Cell Mechanics. | Unal M et al. | — | 2014 | → |
| Delivery of continuously-varying stimuli using channelrhodopsin-2. | Tchumatchenko T et al. | — | 2013 | → |
| Parameters for burst detection. | Bakkum DJ et al. | — | 2013 | → |
| Tracking axonal action potential propagation on a high-density microelectrode array across hundreds of sites. | Bakkum DJ et al. | — | 2013 | → |
| A new indirect co-culture set up of mouse hippocampal neurons and cortical astrocytes on microelectrode arrays. | Geissler M et al. | — | 2012 | → |
| Optogenetics: a novel optical manipulation tool for medical investigation. | Yao JP et al. | — | 2012 | → |
| Protection from glutamate-induced excitotoxicity by memantine. | Kutzing MK et al. | — | 2012 | → |
| Stimulus-evoked high frequency oscillations are present in neuronal networks on microelectrode arrays. | Hales CM et al. | — | 2012 | → |
| Sub-millisecond closed-loop feedback stimulation between arbitrary sets of individual neurons. | Müller J et al. | — | 2012 | → |
| Channelrhodopsin-2 localised to the axon initial segment. | Grubb MS et al. | — | 2010 | → |