GnRH neuron firing and response to GABA in vitro depend on acute brain slice thickness and orientation.
- Authors
- Constantin, Stephanie; Piet, Richard; Iremonger, Karl; Hwa Yeo, Shel; Clarkson, Jenny; Porteous, Robert; Herbison, Allan E
- Year
- 2012
- Journal
- Endocrinology
- PMID
- 22719049
- DOI
- 10.1210/en.2012-1126
The GnRH neurons exhibit long dendrites and project to the median eminence. The aim of the present study was to generate an acute brain slice preparation that enabled recordings to be undertaken from GnRH neurons maintaining the full extent of their dendrites or axons. A thick, horizontal brain slice was developed, in which it was possible to record from the horizontally oriented GnRH neurons located in the anterior hypothalamic area (AHA). In vivo studies showed that the majority of AHA GnRH neurons projected outside the blood-brain barrier and expressed c-Fos at the time of the GnRH surge. On-cell recordings compared AHA GnRH neurons in the horizontal slice (AHAh) with AHA and preoptic area (POA) GnRH neurons in coronal slices [POA coronal (POAc) and AHA coronal (AHAc), respectively]. AHAh GnRH neurons exhibited tighter burst firing compared with other slice orientations. Although Ξ±-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) excited GnRH neurons in all preparations, Ξ³-aminobutyric acid (GABA) was excitatory in AHAc and POAc but inhibitory in AHAh slices. GABA(A) receptor postsynaptic currents were the same in AHAh and AHAc slices. Intriguingly, direct activation of GABA(A) or GABA(B) receptors respectively stimulated and inhibited GnRH neurons regardless of slice orientation. Subsequent experiments indicated that net GABA effects were determined by differences in the ratio of GABA(A) and GABA(B) receptor-mediated effects in "long" and "short" dendrites of GnRH neurons in the different slice orientations. These studies document a new brain slice preparation for recording from GnRH neurons with their extensive dendrites/axons and highlight the importance of GnRH neuron orientation relative to the angle of brain slicing in studying these neurons in vitro.
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| Spike and Neuropeptide-Dependent Mechanisms Control GnRH Neuron Nerve Terminal Ca<sup>2+</sup> over Diverse Time Scales. | Iremonger KJ et al. | β | 2017 | β |
| Electrophysiology of Hypothalamic Magnocellular Neurons In vitro: A Rhythmic Drive in Organotypic Cultures and Acute Slices. | Israel JM et al. | β | 2016 | β |
| Galanin Activates G Protein Gated Inwardly Rectifying Potassium Channels and Suppresses Kisspeptin-10 Activation of GnRH Neurons. | Constantin S et al. | β | 2016 | β |
| Stress and Sucrose Intake Modulate Neuronal Activity in the Anterior Hypothalamic Area in Rats. | Mitra A et al. | β | 2016 | β |
| Differential regulation of GnRH secretion in the preoptic area (POA) and the median eminence (ME) in male mice. | Glanowska KM et al. | β | 2015 | β |
| Morphological Characterization of the Action Potential Initiation Segment in GnRH Neuron Dendrites and Axons of Male Mice. | Herde MK et al. | β | 2015 | β |
| Multitasking in Gonadotropin-Releasing Hormone Neuron Dendrites. | Iremonger KJ et al. | β | 2015 | β |
| Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche. | Perry JR et al. | β | 2014 | β |
| The role of GABA in the regulation of GnRH neurons. | Watanabe M et al. | β | 2014 | β |
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