Understanding cannabinoid psychoactivity with mouse genetic models.
- Authors
- Mackie, Ken
- Year
- 2007
- Journal
- PLoS biology
- PMID
- 17927451
- DOI
- 10.1371/journal.pbio.0050280
- PMCID
- PMC2001210
Thanks to recent advances in the cannabinoid field, investigators have begun to relate cannabis's cellular mechanisms to its psychoactive behavioral effects.
CB1 Expression Levels Vary Widely in the Mouse Hippocampal Formation.In cortex, CB1 mRNA is highly expressed in a very restricted set of interneurons and more moderately expressed in a broader population of principal neurons. A representative coronal section through the hippocampal formation of mouse brain is shown. Nonradioactive in situ hybridization was used to identify CB1 mRNA-containing cells. The very highest levels (darkest red) are found in a restricted subset of cells (arrowheads) that are distributed throughout the hippocampal formation. Other studies have shown these to be CCK-containing GABAergic interneurons. Intermediate levels (light pink) are found in the principal neurons of CA1 (arrows) and CA3. Dentate granule cells (asterisks) do not express CB1 mRNA and provide a measure of the background inherent in this technique. Nuclei are identified by toluidine blue counterstaining. This general pattern of expression is recapitulated throughout the neocortex and amygdala. mo, molecular layer; po, polymorphic layer; sg, granule cell layer; slm, stratum lacunosum; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. (Original image provided by Martin HΓ€ring.)
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