variancePartition: interpreting drivers of variation in complex gene expression studies.

Analysis workflow of gene expression data and meta-data. Standard analysis consists of interpreting gene expression data with respect to variables in the metadata using genome-wide analysis such as a principal components analysis and b hierarchical clustering, and gene-level analysis such as c differential expression. The variancePartition workflow uses a rich statistical framework in the form of a linear mixed model and produces gene-level results and a genome-wide summary to simultaneously interpret gene expression data in the context of multiple variables in the metadata. The workflow produces d gene-level results quantifying the contribution of each metadata variable to the variation in expression of each gene, and e a violin plot to summarize the genome-wide trend and rank the total contribution of each variable. f The gene-level results can be used to identify genes that show high expression variation across individuals (i.e. gene385) or tissue (i.e. gene644). Furthermore, variancePartition facilitates examination of specific genes, and integrating external data enables further interpretation of the drivers of expression variation

Analysis of GEUVADIS dataset identifies drivers of expression variation. a Total variance for each gene is partitioned into the fraction attributable to each dimension of variation in the study. b Violin and box plots of percent variation in gene expression explained by each variable. Three representative genes and their major sources of variation are indicated. c Boxplot of UTY expression stratified by sex. d Boxplot of CCDC85B expression stratified by lab. Inset shows scatter plot of percent GC content versus percent variance explained by lab. Red line indicates linear regression line with coefficient of determination and p-value shown. e Boxplot of ZNF470 expression stratified by individual for a subset of individuals with at least 1 technical replicate. Inset illustrates a cis-eQTL for ZNF470 where expression is stratified by genotype at rs2904239. f Probability of each gene having a cis-eQTL plotted against the percent variance explained by individual. Dashed lines indicate the genome-wide average probability (i.e. 18% of genes have a detected eQTL in this dataset), and curves indicate logistic regression smoothed probabilities as a function of the percent variance explained by individual. Points indicate a sliding window average of the probability of genes in each window having a cis-eQTL. Window size is 200 genes with an overlap of 100 genes between windows. The p-value indicates the probability that a more extreme coefficient relating the eQTL probability to percent variation explained by individual is observed under the null hypothesis

Analysis of Sequencing Quality Control (SEQC) dataset decouples sources of technical variation. a Violin and box plots of percent variation in gene expression explained by each variable. b Boxplot of percent variance explained by RNA sample for human genes and External RNA Controls Consortium (ERCC) spike-in controls. P-value is from one-sided Mann-Whitney test. c Scatter plot of percent GC content and percent variance explained by laboratory. Red line indicates linear regression line with regression coefficient, coefficient of determination and p-value shown

Analysis of ImmVar dataset interprets multiple dimensions of expression variation. a Violin and box plots of percent variation in gene expression explained by each variable. b Principal components analysis of gene expression with experiments colored by batch. c Total variance for each gene is partitioned into the fraction attributable to each dimension of variation in the study design. d Expression of UTY stratified by sex. e Expression of TLR4 stratified by cell type. f Expression of GSTM1 stratified by individual. g Scatter plot of percent GC content and percent variance explained by batch. Red line indicates linear regression line with regression coefficient, coefficient of determination and p-value shown. h Results from variancePartition analysis allowing the contribution of individual to vary in each cell type. i Probability of each gene having a cis-eQTL plotted against the percent variance explained by individual within each cell type. Dashed lines indicate the genome-wide average probability, and curves indicate logistic regression smoothed probabilities as a function of the percent variance explained by individual within each cell type. Points indicate a sliding window average of the probability of genes in each window having a cis-eQTL. Window size is 200 genes with an overlap of 100 genes between windows. The p-value indicates the probability that a more extreme coefficient relating the eQTL probability to percent variation explained by individual is observed under the null hypothesis

Analysis of GTEx dataset identifies drivers of expression variation at multiple levels. a Violin and box plots of percent variation in gene expression explained by each variable. b Results from variancePartition analysis allowing the contribution of individual to vary in each tissue. c Probability of each gene having a cis-eQTL plotted against the percent variance explained by individual within each tissue. Dashed lines indicate the genome-wide average probability, and curves indicate logistic regression smoothed probabilities as a function of the percent variance explained by individual within each tissue. The p-value indicates the probability that a more extreme coefficient relating the eQTL probability to percent variation explained by individual is observed under the null hypothesis. d Fraction of variation in GLMP explained by each source of variation. e GLMP has a cis-eQTL active in blood but not skin