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Chunk #62 — EXPERIMENTAL PROCEDURES — Tissue dissection and expression profiling

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The neuronal transporter gene SLC6A15 confers risk to major depression.
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Frozen brains were sectioned at the level of the dorsal hippocampus and the subregions CA1 and dentate gyrus were laser-microdissected using a laser capture microscope (P.A.L.M. Microlaser Technologies, Bernried, Germany). Extracted RNA was quality checked on the Agilent 2100 Bioanalyser, subjected to two rounds of linear amplification and hybridised to Illumina MouseRef-8 v1.0 Expression BeadChips according to the manufacturer’s protocol (see also supplemental methods). The data discussed in this publication have been deposited in NCBIs Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) and are accessible through GEO Series accession number GSE112211.