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Chunk #61 — EXPERIMENTAL PROCEDURES — Chronic stress paradigm

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The neuronal transporter gene SLC6A15 confers risk to major depression.
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The chronic social stress procedure was performed as described previously (Schmidt et al., 2007; Sterlemann et al., 2008) (see fig. S5 and supplemental methods). In this paradigm, mice are exposed to a highly unstable social and hierarchical situation during their adolescence and early adulthood. After the seven weeks stress procedure all animals were single housed for 5 weeks and then sacrificed under basal conditions.