We identified associations at four loci that were near genome-wide significance in the discovery cohort and had not been associated with stroke in previous studies: SNPs in the ERRF11 and NAA25 (C12orf30) genes with all ischaemic stroke, a SNP in ALKBH8 with large-vessel stroke, and rs13407662 on chromosome 2p16.2 in an intergenic region with small-vessel disease. We took these four forward, with an additional eight of the strongest associations that had not reached genome-wide significance, to replication in an independent sample. None of the associations replicated. Our replication sample contained a cohort of patients of Pakistani ancestry, but, restriction of our analysis to individuals of European ancestry did not alter the results.
