We retrieved TF binding profiles as PFMs from Lai et al. (30) for the PBM experiments, from CIS-BP (4) for TFs in insects, nematodes, and plants, from Bass et al. for worms (31), and from the UNVALIDATED collection of the JASPAR 2022 release (9) (Supplementary Table S1). We processed ChIP-seq, ChIP-exo, and DAP-seq datasets from GTRD (29) and ChIP-exo data from Lai et al. (30) using the RSAT peak-motifs tool (32) to identify enriched motifs (as PFMs) in the corresponding peak sets (Supplementary Table S1 and Supplementary Text for dataset and method details). Our expert curators manually selected the PFMs supported by orthogonal evidence from the literature to either add them to or update former TF binding profiles in the JASPAR CORE collection. The PFMs deemed high quality, but for which our curators did not find any orthogonal support in the literature were added to the JASPAR UNVALIDATED collection. We complemented the JASPAR CORE collection with 329 TF binding profiles and updated three existing profiles with new PFMs (Table 1 and Figure 1). We identified orthogonal support in the literature
