The sample size in the current study was chosen based on our previous experience with similar studies, with a goal of ensuring adequate statistical power while including the least possible number of subjects. Statistical analysis between two groups was performed using two-tailed Student’s t test. Data were expressed as mean ± SEM unless otherwise stated. To compare the means between multiple groups, we employed two-way ANOVA which allows testing for interactions between two factors. Proportions in events in different groups were assessed using the chi-square test; rejection of the null hypothesis (that proportions are the same across groups) indicated that proportion is different in at least one group.
