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Chunk #14 — Results

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GABA-A and NMDA receptor subunit mRNA expression is altered in the caudate but not the putamen of the postmortem brains of alcoholics.
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The demographic characteristics of individuals in this study are shown in Table S2. There was no significant difference in age, PMI, brain pH, RQI, and proportions of smokers and non-smokers between individuals with or without alcohol dependence (Table S1). Expression of the four AMPA (GluA1-4), five kainate (GluK1-5), seven NMDA (GluN1, 2A-D, 3A-B), two delta (GluD1-2), and nineteen GABA-A (α1–6, β 1–3, γ1–3, δ, ε, θ, π, ρ 1–3) receptor subunit mRNAs were quantified by RT-qPCR in the samples collected from the caudate and putamen. The primers designed for GABA-A and glutamate receptor subunits covered all the transcripts available today for the particular subunit (Table S3).