Primary cortical neurons were isolated from newborn wildtype mice as described 36 with modifications. Briefly, cortices were dissociated by papain (10 U/ml, with 1 μM Ca2+, and 0.5 μM EGTA) digestions and plated on Matrigel coated circle glass coverslips (Ø 11 mm). The neurons were cultured in vitro in growth media consisting of: MEM (Invitrogen) supplemented with B27 (Invitrogen), glucose, transferrin, FBS and Ara-C (Sigma).
