HEK293T cells were cultured in DMEM (GIBCO, Cat# 12430047) supplemented with 10% FBS (GIBCO, Cat#16000044) and 1× penicillin-streptomycin (GIBCO, Cat#15140148) and maintained at 37 °C in humidified air containing 5% CO2. HEK293T Transfections were performed using Polyetherimide (fushen biotechnology, Cat#FSF0001).
