Tamoxifen (sigma, Cat# T5648) was dissolved in corn oil at a concentration of 20 mg/ml and stored at −20 °C. Neonatal mice were administrated with tamoxifen through subcutaneous injection in the back of pups at a dosage 40 μg/g (gram, body weight) at a single injection daily for two constitutive days from P3-P4. Young adult mice acquired daily tamoxifen injection through i.p. injection at a dosage of 125 μg/g (gram, body weight) at a single injection daily for three constitutive days.
