Chunk #45 — Methods — BrdU assay

Source: The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents.
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For the proliferation assay, mice were pulsed with 100 μg/g (gram, body weight) of BrdU at 2 h before sacrificed. For differentiation assay, BrdU was administrated into drinking water with 1 mg/ml for 14 days.