HEK 293 T Cells were transfected with pGL3-Hes1-Luc or pGL3-Sox11-Luc reporter constructs, β-galactosidase (β-gal) expression vectors and other relevant plasmids. The promoter of Sox11 we cloned is -1kb to 0 (TSS), where Olig2 binds as shown in ChIP-seq result. At 36 h post transfection, cells were harvested and luciferase activities were measured in triplicate and normalized to internal β-gal activity to normalize for transfection efficiency.
