In addition, we accessed RNA-seq data generated for iPSC-derived neurons from the Broad iPSC study [42] (synapse ID: syn3607401). Forebrain neurons from wild-type background were generated using an embryoid body-based protocol to produce neural progenitor cells (day 17) and mature neurons (days 57 and 100). RNA was purified using a PureLink RNA mini-kit (Life Technologies). RNA-seq libraries were prepared using Illumina Strand Specific TruSeq protocol and sequenced to obtain an average of 75 M reads in paired reads per sample.
