over 5–7 days. The resulting neural rosettes were then isolated by enzymatic selection (StemCell Technologies) and cultured as neural progenitor cells (NPCs). NPCs were then differentiated by culturing in neural maturation medium (neurobasal medium supplemented with B27, GDNF, BDNF, cAMP). RNA was collected from the cells and sequenced following the same protocol and processing pipeline as the DIAN and Knight-ADRC dataset.
