Analysis of the spike shape for the ‘hyper’ vs ‘hypo’ groups revealed that the spike amplitude increased by 53% in the controls (P<0.0001), 30% in LRs (P<0.0001) and 55% in NRs (Figure 5h and Supplementary Figure 2e). A big change was observed in the 5-ms AHP when comparing the ‘hyper’ and ‘hypo’ neurons in all three groups. Controls exhibited an increase of 196% (P<0.0001), LRs 123% (P<0.0001) and NRs 85% (P<0.0001, Figure 5i and Supplementary Figure 2f). The spike width of the ‘hyper’ neurons was narrower than that of the ‘hypo’ neurons (−60% in control P<0.0001, − 46% in LR P<0.0001 and − 43% in NR P<0.0001) (Figure 5j and Supplementary Figure 2g). The threshold for firing was less depolarized in the ‘hyper’ vs ‘hypo’ neurons (3.3 mV for control neurons P = 0.0012, 3.8 mV for LR neurons P = 0.005 and 5.6 mV for NR neurons P<0.0001), with the NR neurons having a more depolarized threshold in both the ‘hyper’ and ‘hypo’ groups (Figure 5k and Supplementary Figure 2h).
