To determine how this EHR-based approach overlaps with previously published methodologies, we evaluated previously identified OUD-associated SNPs (Table 4)(Cheng et al., 2018; Hancock et al., 2015; Nelson et al., 2016; Polimanti et al., 2020). We tested SNPs from four previous studies in our sample. Of these, SNP rs3778150 was significantly related with opioid addiction in study that included European Americans, Australia and African Americans(Hancock et al., 2015). The SNP is close to the OPRM1 gene, which encodes one of three opioid receptors in humans. In our sample, rs3778150 was significantly associated with OUD (p=0.012) in the same direction as previously reported. Other tested SNPs were not significantly associated with prevalence of OUD. All these SNPs were also tested in the sub-group analysis with exposed controls, and similar p values were observed (Table 4).
