Chunk #58 — II. Samples for genome studies of human addiction vulnerabilities and related phenotypes (Figure 2)

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Molecular genetics of addiction and related heritable phenotypes: genome-wide association approaches identify "connectivity constellation" and drug target genes with pleiotropic effects.

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Genotyping: Allele frequency data was assessed in 16 DNA pools, each containing equal amounts of DNA from 60 individuals using a single set of 49 arrays that assessed 2,427,354 SNPs. Methods used for these samples revealed r = 0.85 overall correlations between pooled and individual genotyping for all SNPs. However, there were much more modest, r = 0.58 correlations between dependent vs nondependent data derived from pooled vs individual genotyping for the SNPs that displayed nominally-significant differences. Individual genotyping followed up nominally-positive results for 39,213 SNPs in 1,050 dependent and 879 nondependent smokers. The convergence analyses presented here use data from the subset of these 39,213 SNPs that lie within genes.