We compared PPP3CA expression in brain samples from 57 pathologically verified AD patients and 15 non-AD control using real time PCR/Taqman assays. We focused on PPP3CA isoforms with intact functional domains, namely CNEX1-3, 1-4, 13-14, and 13-15. There was significant reduction of levels of CNEX1-3, 1-4, and 13-14 isoforms in medial temporal gyrus. Reduction in CNEX1-4 and CNEX13-14 expression also reached significance in mRNA from medial frontal gyrus samples (Figure 5). Expression of the CNEX13-15 isoform, by contrast, was not significantly reduced in medial temporal gyrus and was actually significantly increased in AD medial frontal gyri (Figure 5, Mann-Whitney p = .032).
