We found nine molecularly distinct neuron types. Although enteric neurons are commonly divided into nitrergic and Calretinin-expressing subtypes, our data indicate that the more natural split is between nitrergic (i.e., expressing the neuronal nitric oxide synthase Nos1, ENT1-3) and cholinergic (i.e., expressing Chat and Slc5a7, ENT4-9) neurons. The nitrergic ENT1-3 expressed the high-affinity choline transporter Slc5a7, but Chat was very low or undetected in those neurons. In addition, ENT7 and ENT8 co-expressed Slc17a6 (VGLUT2). Many enteric neurons also expressed a variety of neuropeptides (Figure S4), including Gal, Cartpt, Nmu, Vip, Cck, and Tac1. A more detailed analysis of these neurons will be published elsewhere (U.M., unpublished data).
