The enteric nervous system has not been previously studied in molecular detail using single-cell methods. Here, we report on the composition of the myenteric plexus of the small intestine, whereas we did not include cells from the submucosal layer or other regions of the gastrointestinal tract. Based on marker gene expression, morphology, location, and projection targets, approximately ten cell types have been previously described (Furness et al., 2014, Qu et al., 2008) in the myenteric plexus of the mouse.