This study had several strengths, including a large sample size, identical measures and participant characteristics to the first cohort, and measures that were repeatedly collected across the BrAC curve. However, some limitations should also be noted. Because the main purpose of the study was to examine alcohol response in a high-risk population, the second cohort of subjects only included HD. Therefore, it is unclear whether LD’s subjective and objective responses to alcohol are also reproducible. Since this replication study was performed within the same laboratory as the original (though with a different research staff), unintentional experimenter biases may have influenced the results in both studies. Therefore, a direct replication attempt by an outside lab should be performed in order to remove such potential confounds. Finally, as previously described, one notable difference between the two studies was the lack of a third session administering a subthreshold dose of alcohol to participants of the second sample. While we found no significant order effects in our previous papers, future studies should attempt to characterize acute responses to various doses of alcohol in HD,
