To test whether brain regions work together differently in youth with a positive FH of AUD, models of brain activity during SWM were created. The OA model was developed to include brain regions that best approximate patterns previously reported for older adolescents and adults in response to an SWM fMRI task (39-44)(Figure 1). Due to the constraints of the analytic approach, the model was made recursive (and thus unidirectional) to maximize our ability to test our hypotheses. Regions of interest (ROIs) included: 1) right inferior parietal lobule, 2) right superior parietal lobule, 3) right middle frontal gyrus, and 4) left middle frontal gyrus, based on evidence that these regions are a) integral to SWM functions (39-44), b) sensitive to shifts in cortical organization or change in strategies that accompany adolescent development (31-36, 45, 46), and c) susceptible to FH effects (30, 47, 48).
