drawn from the 22,283 transcripts assayed by the Affymetrix U133A microarray, the 5.08-fold NF-κB/GRE skew observed in the promoters of differentially expressed genes was substantially greater than expected by chance under the genome-wide null distribution (Figure 2; two-tailed p = 0.0070). Similar results emerged in analyses estimating reciprocal NF-κB/GRE skew from the high-stringency 5% FDR criterion for differential gene expression (p = 0.014). Thus, the inverse correlation between NF-κB/Rel and GRE TFBM prevalence in promoters of isolation-linked genes likely reflects trans-activational influences rather than a population-level bias in the cis-structure of human promoters.
