Bioinformatic indications of a simulatneous decrease in transcription of GR target genes (.53-fold difference) and an increase in transcription of NF-κB/Rel target genes (2.69-fold difference) is consistent with the known cross-inhibition of those two pathways [37]. Combined TELiS analysis of both pathways yielded a net 5.08-fold skew in promoter TFBM distributions (ratio = 2.69/0.53). To ensure that this skewed motif prevalence reflected the effects of reciprocal signaling in trans, rather than an inverse relationship between NF-κB versus GRE TFBMs in the cis-regulatory structure of the human gene population, we assessed the whole-genome distribution of NF-κB/GRE prevalence ratios by computing TFBM prevalence ratios in a similarly sized random sample of all assayed genes (rather than the specific subset showing differential expression in social isolates). Relative to the distribution of cis-structural TFBM prevalence ratios generated by 10,000 random samples of 209 transcripts drawn from the 22,283 transcripts assayed by the Affymetrix U133A microarray, the 5.08-fold NF-κB/GRE skew observed in the promoters of differentially expressed genes was substantially greater than expected by chance under the genome-wide null distribution (Figure 2; two-tailed p =
