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Chunk #16 — Methods — Morris water maze spatial and reversal learning in adults — Initial Learning Acquisition

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Adolescent binge drinking alters adult brain neurotransmitter gene expression, behavior, brain regional volumes, and neurochemistry in mice.
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Mice were evaluated in the hidden platform task using the same procedure as described above. The platform was submerged under the water in Quadrant 1. C57BL/6 mice have been found to acquire learning by day six of testing (Moy et al., 2007). Mice were given four trials per day to learn the position of the hidden platform. Once the 15 second criterion was reached for the entire group, mice were given a one-minute probe trial in the pool without the platform in place. During the probe trial, the time spent in each quadrant of the pool was measured.