(Assay ID 4326319E), human ACTB (Assay ID 4310881E), or mouse Actb (Assay ID 4352341E) was used as the reference gene as appropriate. For Vkorc1, the primers F: 5′-GCCCTCTCACTGTACGCACT-3′ and R: 5′-CCCACCGAGAGGAGAAGAC-3′ were used with SYBR Green (Thermo Fisher Scientific). For Ube2l3, the primers F: 5′-GGCTGATGAAGGAGCTTGAA-3′ and R: 5′-CAGGAACAATAAGCCCTTGC-3′ were used with SYBR Green. Each 10 μL qPCR reaction contained 1 μL cDNA (diluted 1:3 with water) and was performed in technical duplicate or triplicate. Reactions were carried out on the ViiA 7 Real-Time PCR system or the 7900HT Fast Real-Time PCR System (Thermo Fisher Scientific). Relative expression levels were quantified by the 2−ΔΔCt method.
