To study GABAB1 splice junction changes in alcoholics, we calculated RPJM for each junction. The RPJM calculation also considered the intron and intergenic regions which is critical given that many unknown splice junctions and mapped reads were found at these regions. Among significantly changed RPJMs in the genome wide level, 11 differentially expressed GABAB1 junctions were found (Figure 7) including 10 known and one novel splice junction(s) (Table S5 in Supplement 1).
