For RT-PCR, RNA was isolated from P8 and P90 SVZ tissue or from FACS-purified SVZ cells (WT and CNP-hEGFR mice), using Trizol (Invitrogen). RNA (1μg) from each sample was reverse-transcribed using the SuperScript™ First-Strand cDNA Synthesis kit (Invitrogen). The mouse gene-specific primers were obtained from Integrated DNA Technologies, Inc. (Coralville, IA). Primer sequences for PCR analysis are found in Supplementary Experimental Procedures. Genes were amplified by denaturation at 94°C for 1 min, annealing at 60°C for 1 min, and extension at 72°C for 1 min for 28 cycles. PCR products were resolved by 1.2% agarose gel electrophoresis and visualized by ethidium bromide staining.
