GEArray™ expression array systems (cat #OMM-404MM and #OMM-404MM, SuperArray, Bethesda, MD) consisting of spotted cDNA fragments encoding 250 mouse genes involved in NSC development/neurogenesis and in Nocth signaling, as well as control sequences (PUC18, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), peptidylpropyl isomerase A (Ppia), and b-actin) were employed to compare gene expression between WT and CNP-hEGFR SVZ tissue. Total RNA was isolated with theTrizol method (Invitrogen) and further processed for microarray hybridization according to the manufacturer’s instructions. The arrays were visualized by autoradiography and hybridization signals were scanned and analyzed for density in GEArray Expression Analysis Suite 2.0. The normalized value for each gene was calculated by dividing the value of each gene by the average value of the housekeeping genes GAPDH, Ppia, and β-actin.
