we took forward the most significant 96 SNPs for replication. Based on power estimations, after adding 30,000 samples in stage 2 these variants had a priori Power >=85% to reach P= 5 × 10−8 in the meta-analysis. Loci were considered independent when separated by at least 1 Mb down and upstream of the top GWAS signal. The 96 variants included the 82 index SNPs representing each of the 82 loci reaching P<5×10−6 in Stage 1, 9 SNPs that lie within the same 2Mb windows as the 82 but which were independent from the main signal (secondary signals), and the top-five most associated SNPs of the X-chromosome (with P <5×10−5).
