Human haplotypes for Nigeria (YRI) and Utah (CEU) samples in CRH gene region plus 5 Kb upstream and 1 Kb downstream were downloaded from HapMap project Release 21 (http://www.hapmap.org/). The following markers were used in our analyses (Fig 2, in 5′-3′ orientation: rs10098823, rs5030877, rs7839698, rs5030875, rs4501563, rs6472257, rs6999780, rs12721507, rs12721508, rs12721509, rs3176921, rs12721510, rs6158, rs12721511, rs7459924, rs6982394, rs11986876, and rs11997816). Based on Manhattan distances weighted by minor allele frequency and marker average LD, haplotypes for rhesus macaque (five most common) and human were clustered hierarchically using R (http://www.r-project.org). The ancestral sequences at each SNP site were estimated as described by Pollard et al. 23 with multiple alignments of human (March 2006 assembly), chimp (Mar 2006 assembly), and rhesus macaque (January 2006 assembly) from UCSC Genome Browser (http://genome.ucsc.edu/).
