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Chunk #3 — METHODS — FOCUSED RESEQUENCING

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CRH haplotype as a factor influencing cerebrospinal fluid levels of corticotropin-releasing hormone, hypothalamic-pituitary-adrenal axis activity, temperament, and alcohol consumption in rhesus macaques.
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DNA samples from two other rhesus macaque colonies (AlphaGenesis, N = 69; U. of Wisconsin, N = 94 - the kind gift of M. Schneider) were screened for alleles thought to co-segregate with –2232 G, based on our initial sequencing. Focused re-sequencing was also performed on DNA from NIHAC animals of known –2232 C/G genotype (C/C:N=40; C/G: N=60) ) to verify that rhCRH-H2 variants were in allelic identity.