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Chunk #4 — METHODS — FOCUSED RESEQUENCING

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CRH haplotype as a factor influencing cerebrospinal fluid levels of corticotropin-releasing hormone, hypothalamic-pituitary-adrenal axis activity, temperament, and alcohol consumption in rhesus macaques.
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Various other primate samples (New World monkey- Ateles geoffroyi; Old World Monkey – Macaca nemestrina, Macaca fascicularis, Papio hamadryas, Papio anubis; Ape – Pan troglodytes) were sequenced for determination of sequence identity in the region surrounding the -2232 C/G site. DNA samples from 10 wild baboons (Papio hamadryas) from the Awash National Park, Ethiopia were the kind gift of J. Phillips-Conroy and C. J. Jolly. Additional nonhuman primate samples were obtained from the Coriell Institute for Medical Research. Alignments of CRH and 5′ flanking region sequences were performed using ClustalW and/or DNASIS-Mac V.2.0 software. Sequence identity percentages for interspecies comparisons were calculated using the web-based pairwise alignment program EMBOS (http://www.ebi.ac.uk/emboss/align/) using the default parameters.